Raising of Rabbit Hyperimmune Serum for Laboratory Diagnosis of Peste Des Petits Ruminants (ppr) Virus
نویسنده
چکیده
Agar gel immunodiffusion (AGID) test is a simple and rapid method for the diagnosis of Peste des petits ruminants (PPR) which requires the preparation of potent specific hyperimmune serum. This study deals with the raising of hyperimmune serum against PPR virus in rabbit. Four different inocula included PPR vaccine reconstituted in phosphate buffer saline, PPR antigen suspended in phosphate buffer saline, PPR vaccine in oil adjuvant and PPR antigen in oil adjuvant were used. All inocula were inoculated at different intervals and concluded that inoculum contained PPR antigen in oil adjuvant provoked better immune response against PPR virus in rabbits.
منابع مشابه
Rapid competitive enzyme-linked immunosorbent assay for detection of antibodies to peste des petits ruminants virus.
Peste des petits ruminants (PPR) is a contagious viral disease of small ruminants that is of economic importance in Africa, the Middle East, and Asia. We developed a rapid competitive enzyme-linked immunosorbent assay (rapid c-ELISA) for the diagnosis and surveillance of PPR. This assay detects PPR virus (PPRV) antibodies in serum samples by quantifying the amount of monoclonal antibody (MAb) P...
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With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
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Peste des petits Ruminants (PPR) is an acute, febrile, highly contagious and economically important viral disease of small ruminants. However PPR is more prevalent in sheep and goat. Competitive ELISA, Virus neutrilization test, and RT-PCR are the available techniques for diagnosis of PPR which give rapid detection where as Agar gel immunodiffusion and Counter immunoelectrophoresis were previou...
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